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With its long-standing tradition as a model organism, many techniques using Drosophila melanogaster have been established and continue to be developed, while the recent invention of techniques allowing the knock-down of genes by RNA interference and gene replacement by homologous recombination demonstrates the ongoing efforts to broaden the technical repertoire. In Drosophila: Methods and Protocols, leading experts contribute a collection of protocols covering standard techniques as well as novel methods. As a volume in the highly successful Methods in Molecular Biology series, the chapters contain brief introductions to the subject matter, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.§Cutting-edge and essential, Drosophila: Methods and Protocols allows novices and experts alike to tap into the power of one hundred years of expertise involving this vital model system.