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In this book, a brief introduction to the bacteria and bacterial concrete in rural road Construction. This experimental and analytical research work is an effort to try to develop the awareness and importance of bacterial concrete and its utilization in a productive manner among the construction professionals. Use of concrete nowadays increases rapidly in the area of buildings as well as in infrastructures. By incorporating Bacillus Pasteurii bacteria, certain properties of concrete like compressive strength and durability can be improved. This unique way of concrete design crossbreed between biology and engineering study of concrete is called microbial concrete, which involves the utilization of bacteria to increase the strength and durability of concrete. CC pavement is designed for a Rural Road as 500 cvpd at the end of the construction period. Hence an experimental research study has been conducted for comparing and studying the effect of Bacillus Pasteurii bacteria in concrete for rural road rigid pavements construction.